My experience is exactly opposite.
Sure a big line is initially discouraging. But while in line, if I've chosen one of several lines, I fixate: is my chosen cashier a slow idiot? is a customer ahead of me fumbling for change? Should I jump? There's another line opening, should I get ready to run for it? I invariably end up at the front more directly annoyed and snippy at "my" cashier and the whole process. With a single line, I can see everything humming along, moving forward more often, if one transaction takes a while, so what, it's not a race, I relax more, I'll get there.