The original version of this guide was a great introduction to jQuery for me (thanks!). Overall this looks like a much easier and more interactive way to go through the material.
I do have one comment, though: After reading through the single-page guide, I went back to it for a few weeks and often used Ctrl-F to find specific references to things I couldn't exactly remember how to do, and I don't see a way to search similarly in the new version. Ideally the improved navigation should eliminate the need to use such a blunt tool, but I still wonder if someone might want to come back and find something, remembering some random syntax but not remembering where it was. Not sure exactly how you would fix that in the new guide, or how serious it is, so it's just some food for thought.